Analysis of intercellular lipid lamellae in the stratum corneum after smoke application (barrier damage)

Anti-Pollution Matrix > Methods > Method list > Analysis of intercellular lipid lamellae in the stratum corneum after smoke application (barrier damage)

Method

Cigarette smoke is used as a model for the effect of chemical noxe caused by air pollution (e.g. combustion of organic substances) on human skin.

A defined amount of fresh cigarette smoke is applied directly to the skin (forearm) in a smoke chamber. After standardized smoke application, sampling is performed 24h after "smoking". Sampling is performed using Lipbarvis® tear-offs; 2 tear-offs are taken per test area. The analysis of the quality of the epidermal barrier in the stratum corneum is performed by means of analysis in the transmission electron microscope and by morphometric evaluation [1-3].

In order to obtain reproducible results and a good differentiation of active products, about 12 to 15 test persons are necessary. The results are compared with a non-smoked area. The method is very sensitive and often shows results that are not yet detectable by TEWL and skin moisture.

 

Detection of

• Structure and quality of epidermal barrier lipids in the stratum corneum, Quantitative determination of lipid lamellae in the intercellular space (barrier integrity)

 

Suitable for

• Caring products which, due to their caring properties, show protective effects on the skin barrier and its lipids (Protect).
• Caring products which, due to their caring property, show restorative action on the damaged skin barrier and its lipids (Repair).

 

Literature

[1] D.Dähnhardt, C.Surber, S.Dähnhardt-Pfeiffer (2018) "Influence of Topical Formulations: Lipid Lamella Organization and Lipid Composition of Stratum Corneum as a Surrogate Marker for Barrier Integrity. Surber C, Abels C, Maibach H (eds): pH of the skin: issues and challenges. Curr Probl Dermatol. Basel, Karger, 2018 vol 54, pp 166-172 DOI: 10.1159/000489530
[2] S. Daehnhardt-Pfeiffer, C. Surber, K.-P. Wilhelm, D. Daehnhardt, G. Springmann, M. Boettcher, R. Foelster-Holst (2012) "Noninvasive Stratum Corneum Sampling and Electron Microscopical Examination of Skin Barrier Integrity: Pilot Study with a Topical Glycerin Formulation for Atopic Dermatitis." Skin Pharmacol Physiol (2012) 25:155-161 DOI: 10.1159/000336789
[3] D. Ditgen, D. Segger, J. Klaucke, A. von Seebach, D. Dähnhardt, S. Dähnhardt-Pfeiffer , D. Westphal, J. Degwert: (2017) Effects of cigarette smoke on the skin in comparison to UV radiation: Parallels and differences - Usage of the smoke chamber model for anti-pollution claim support - Poster contribution SEPAWA Berlin.