Anti-Pollution Matrix EN – Methods – Method list – (Differential) tape stripping

Anti-Pollution Matrix

(Differential) tape stripping

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> (Differential) tape stripping


Tape stripping is a standard method for investigating the penetration kinetics of topically applied substances into the skin. Here, parts of the stratum corneum (SC) are removed layer by layer. Adhesive film strips are applied one after the other and removed from the skin. In this way, layers of corneocytes and a certain amount of topically applied substances are removed.

For the calculation of the penetration profile, it is necessary to determine the amount of substances and the amount of SC removed with a single strip of tape. The topically applied substances that are removed from the skin can be determined using classical analytical methods such as high-pressure liquid chromatography, mass spectroscopy and spectroscopic measurements. The amount of corneocytes located on the tape strips can be detected by their pseudoabsorption.

These investigations allow the quantification of substances applied to the skin in the SC.

Furthermore, follicular penetration can be determined by differential tape stripping (DTS). The reservoir of the SC is removed by means of tape stripping and then cyanoacrylate is applied. This runs into the hair follicles and polymerizes, so that the follicular portion of a topically applied substance can be recorded separately.


Proof of
  • Penetration of actives, particles (e.g. Particulate matter, Soot), Cosmetics into the skin and generation of a penetration profile.
  • Information on the homogeneity and distribution of formulations on the skin and in the stratum corneum


Suitable for
  • Investigation of the distribution of active ingredients, particles, cosmetics in the skin and creation of a penetration profile



[1] J. Lademann, U. Jacobi, C. Surber, H.J. Weigmann, J.W. Fluhr, The tape stripping procedure - evaluation of some critical parameters, Eur J Pharm Biopharm, 72 (2009) 317-323, DOI: 10.1016/j.ejpb.2008.08.008
[2] A. Teichmann, U. Jacobi, M. Ossadnik, H. Richter, S. Koch, W. Sterry, J. Lademann, Differential stripping: Determination of the amount of topically applied substances penetrated into the hair follicles, Journal of Investigative Dermatology, 125 (2005) 264-269. DOI: 10.1111/j.0022-202X.2005.23779.x
[3] U. Jacobi, N. Meykadeh, W. Sterry, J. Lademann, Effect of the vehicle on the amount of stratum corneum removed by tape stripping, J Dtsch Dermatol Ges, 1 (2003) 884-889. DOI: 10.1046/j.1439-0353.2003.03733.x
[4] P. Dong, V. Nikolaev, M. Kroger, C. Zoschke, M.E. Darvin, C. Witzel, J. Lademann, A. Patzelt, M. Schafer-Korting, M.C. Meinke, Barrier-disrupted skin: Quantitative analysis of tape and cyanoacrylate stripping efficiency by multiphoton tomography, Int J Pharm, 574 (2020) 118843. DOI: 10.1016/j.ijpharm.2019.118843