In vivo Suction Blister Model

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> In vivo Suction Blister Model

Method

The Suction Blister Method serves as an in vivo method for the investigation of various biochemical and molecular biological parameters of human skin.

It enables to mechanically separate the epidermis from the dermis.

Suction chambers connected to a vacuum pump are attached to the skin (forearms/back) with their flat bottom. There are circular openings in the bottom of these attachments. With the aid of a defined negative pressure, a suction process is created on the skin, which leads to the generation of a blister (suction blister) within 2 to 3 hours. This results in a separation of epidermis and dermis at the level of the basal membrane. The interstitial fluid of the suction blister and the epidermis (blister roof) can be removed with sterile instruments and analyzed in vitro.

The Suction Blister Method can be used to investigate an antioxidant product effect after stimulus exposure (e.g. UV irradiation, cigarette smoke as a model air pollutant).

ELISA assays are used to investigate inflammatory parameters and oxidative stress markers in the interstitial suction blister fluid. The blister roofs can be used for immune-histochemical analyses.

 

Detection of

• 8-isoprostane (lipid peroxidation), carbonylated proteins, interleukines, collagen und MMPs in the suction blister fluid (ELISA assays)
• DNA damage from the blister roof (immunohistochemistry)

 

Suitable for

• Leave on products that provide protection from pollution exposure (e.g., sunscreen products, film-forming products).
• Leave on products with antioxidants as active principle

References

• U. Kiistala. Suction blister device for separation of viable epidermis from dermis; J Invest Dermatol. 50(2); 129-137 (1968), DOI: 10.1038/jid.1968.15
• M.R. Barr, S.L. Walker,W.Tsang, G.I. Harrison, P. Ettehadi, M.W. Greaves and A.R. Young. Suppressed alloantigen presentation, increased TNF-α, IL-1, IL-1Ra, IL-10, and modulation of TNF-R in UV-irradiated human skin. J Invest Dermatol. 112(5); 692-698 (1999), DOI: 10.1046/j.1523-1747.1999.00570.x
• K.M. Südel, K. Venzke, E. Knußmann-Hartig, I. Moll, F. Stäb, H. Wenck, K.Wittern, G. Gercken and S. Gallinat. Tight control of matrix metalloproteinase-1 activity in human skin. Photochemistry and photobiology. 78(4); 355 – 360 (2003), DOI: 10.1562/0031-8655(2003)078<0355:tcomma>2.0.co;2
• S. Kuhn, R. Wolber, L. Kolbe, O. Schnorr, H. Sies. Solar-simulated radiation induces secretion of IL-6 and production of istoprostanes in human skin in vivo; Arch Dermatol Res. 297; 477-479 (2006), DOI: 10.1007/s00403-006-0648-2
• K.E. Kim, D. Cho and H.J. Park. Air pollution and skin diseases: Adverse effects of airborne particulate matter on various skin diseases. Life Sci. 152; 126-134 (2016), DOI: 10.1016/j.lfs.2016.03.039