Lipid peroxidation of lipids in the stratum corneum after smoke application

Anti-Pollution Matrix > Methods > Method list
> Lipid peroxidation of lipids in the stratum corneum after smoke application

Method

Cigarette smoke is used as a model for the effect of chemical noxe caused by air pollution (e.g. combustion of organic substances) on human skin.

A defined amount of fresh cigarette smoke is applied directly to the skin (forearm) in a smoke chamber. After standardized smoke application, sampling is performed 24h after “smoking”. Sampling is performed using Lipbarvis® tear-offs; 2 tear-offs are taken per test area. By analyzing the second Lipbarvis® tear-off, not the lipids of the hydrolipid film on the skin surface are analyzed, but the lipids in the intercellular space in the middle stratum corneum. The analysis of the lipids is performed after extraction by HPTLC [1-3].

In order to obtain reproducible results and a good differentiation of active products, about 10 to 15 test persons are necessary. The results are compared with a non-smoked area.

 

Detection of

• Lipid peroxidation of lipids in the stratum corneum, but without the surface lipid film (sebum film)

 

Suitable for

• Care products which, due to their skin care properties, have a protective effect on the skin barrier and its lipids (Protect).
• Care products which, due to their care properties, have a restorative effect on the damaged skin barrier and its lipids (Repair).

 

References

[1] D.Dähnhardt, C.Surber, S.Dähnhardt-Pfeiffer (2018) "Influence of Topical Formulations: Lipid Lamella Organization and Lipid Composition of Stratum Corneum as a Surrogate Marker for Barrier Integrity. Surber C, Abels C, Maibach H (eds): pH of the skin: issues and challenges. Curr Probl Dermatol. Basel, Karger, 2018 vol 54, pp 166-172 DOI: 10.1159/000489530
[2] S. Daehnhardt-Pfeiffer, C. Surber, K.-P. Wilhelm, D. Daehnhardt, G. Springmann, M. Boettcher, R. Foelster-Holst (2012) "Noninvasive Stratum Corneum Sampling and Electron Microscopical Examination of Skin Barrier Integrity: Pilot Study with a Topical Glycerin Formulation for Atopic Dermatitis." Skin Pharmacol Physiol (2012) 25:155-161 DOI: 10.1159/000336789
[3] Effects of cigarette smoke on the skin in comparison to UV radiation: parallels and differences - Usage of the smoke chamber model for anti-pollution claim support. D. Ditgen, D. Segger, J. Klaucke, A. von Seebach, D. Dähnhardt, S. Dähnhardt-Pfeiffer , D. Westphal, J. Degwert: (2017) Poster contribution SEPAWA Berlin.