Microdialysis

Anti-Pollution Matrix > Methods > Method list > Microdialysis

Method

Dermal microdialysis can be used to investigate the dermal penetration, absorption and metabolism of applied agents.

This method is based on diffusion processes that take place through a semi-permeable membrane. The dialysis membrane is inserted into a defined depth of the dermis (ex vivo/ in vivo) using a guide cannula. The membrane is perfused with a carrier solution (perfusate) at a constant flow rate. By means of a syringe pump, this flow rate can be maintained for many hours. Due to the concentration gradient between the interstitial fluid and the perfusate, the body's own molecules as well as potentially topically applied substances diffuse through the membrane into the perfusate, which thus becomes a dialysate. Finally, the obtained dialysate is collected and analyzed with regard to the substances of interest.

In dermatology, the microdialysis technique is currently mainly used to investigate the skin barrier function, the penetration of active substances and how they are metabolized as well as to investigate the release of inflammatory mediators. [1, 2].

 

Proof of

• Penetration of active substances or pollutants such as soot, smoke oder particulate matter into the skin, dermis etc. [3]
• Follicular penetration via defined follicular closure [4]
• Metabolization products

 

Suitable for

• Investigation of substance / compound penetration

 

References

[1] M. Muller, Science, medicine, and the future: Microdialysis, BMJ, 324 (2002) 588-591, doi: 10.1136/bmj.324.7337.588
[2] Y.J. Li, J. Peris, L. Zhong, H. Derendorf, Microdialysis as a tool in local pharmacodynamics, Aaps J, 8 (2006) E222-E235, doi: 10.1007/BF02854892
[3] N. Doge, S. Honzke, F. Schumacher, B. Balzus, M. Colombo, S. Hadam, F. Rancan, U. Blume-Peytavi, M. Schafer-Korting, A. Schindler, E. Ruhl, P.S. Skov, M.K. Church, S. Hedtrich, B. Kleuser, R. Bodmeier, A. Vogt, Ethyl cellulose nanocarriers and nanocrystals differentially deliver dexamethasone into intact, tape-stripped or sodium lauryl sulfate-exposed ex vivo human skin - assessment by intradermal microdialysis and extraction from the different skin layers, Journal of Controlled Release, 242 (2016) 25-34, https://doi.org/10.1016/j.jconrel.2016.07.009
[4] A.L. Klein, M. Lubda, P. Akbarzadeh Taghavi, J. Lademann, I. Beckers, J. von Hagen, H. Kolmar, A. Patzelt, Solvent-Containing Closure Material Can Be Used to Prevent Follicular Penetration of Caffeine and Fluorescein Sodium Salt on Porcine Ear Skin, Skin Pharmacol Physiol, 33 (2020) 117-126, DOI: 10.1159/000505839